TrueFectTM Transfection Reagent

- For DNA transfection


Catalog_No.ProductUnitPrice
NF0866-3TrueFect, Maximum strength for DNA in vitro tranfection, sufficient for 1500-3000 transfection (96 well plate)1.0 mL$169.00



Key Features:

  • Highly effective in DNA transfection with low cytotoxicity
  • Suitable for long DNA
  • Applicable to many cell lines including sf9 cell
  • Applicable to many cell lines
  • Generate lentivirus with extremely high titer
  • Stable formulation with long shelf life

Broad Transfection Spectrum for Mammalian Cell Types:


Cell Lines Transfection Efficiency(% GFP) Cell Lines Transfection Efficiency(% GFP)
3LL 51% K562 22%
B16-F10 72% L929 52%
BAEC 52% MCF-7 51%
BHK-21 86% MDCK 52%
Ca Ski 78% Neuro2A 72%
CaCo2 32% NIH 3T3 88%
CHO 83% PC12 34%
HCS-2/8 42% SH-SY5Y 14%
HEK-293 80-90% SiHa 62%
HeLa 81% SKOV3 52%
HLMEC 50-65% HUVEC 51%
H-MVEC 49% IGROV1 21%
Huh-7D12 22% Jurkat 5%-15%
ATT20 38% 6CSFMEo 73%
SK-N-SH 20% WEHI 231 25%
McArdle 7777 60% SAOS-2 52%
Hep3D 78% SN56 69%
SHEP 71% MC3T3-E1 80%
MDA-MB-231 38% BT474 46%
3T3-442A 10% C2C12 46%
COS-7 60-70% Primary mouse keratinocytes 20-30%
CV-1 53% Primary human pre-adipocytes 20-30%
D 407 62% Primary human skin fibroblasts 15%
DHD Pro.b 51% Primary mouse embryonic fibroblast 10%
LS180 46% Primary melanocyte 15-20%
A549 68% Hela-S3 78%

Primary Murine Skin Fibroblast transfected with pEGFP-C1 plasmid using TrueFectTM In Vitro DNA Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope with DIC phase imaging (left panel) and FITC imaging (right panel) 24 hours post-transfection

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